Methods of low-energy induced fluorescence and spectrophotometry for the study of blood cells

Authors

DOI:

https://doi.org/10.31652/3041-1955/2024-01-02-04

Keywords:

fluorescence, fluorescent probe, low-energy induced fluorescence microscopy, spectrophotometry, blood samples, myeloid leukemia

Abstract

The article describes the implementation of the method of low-energy induced fluorescence microscopy using a laser with a wavelength of 635 nm as a source of radiation and the spectrophotometry method for analyzing the absorption spectra of fluorescent probes. The results of theoretical and experimental studies of blood cells of healthy people and chronic myelogenous leukemia by fluorescence microscopy are presented.

Author Biography

  • Victoria Dumenko, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University

    Victoria Dumenko, Candidate of Science in Engineering, Associate Professor, Department of Physics and Teaching Methods of Physics, Astronomy, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University, 32 Ostrozkyi Str., Vinnytsia 21001, Ukraine

References

Павлов С. В., Кожем'яко В. П., Колесник П. Ф., Козловська Т. І., Думенко В. П. Фізичні основи біомедичної оптики. Вінниця: ВНТУ, 2010. 150 с.

Wentrup R., Winkelmann N., Mitroshkin А., et. al. Photodynamic therapy plus chemotherapy compared with photodynamic therapy alone in hilar nonreselectable cholangiocarcinome. Gut Liver. 2016. Vol 10 (3). Р. 470-475. DOI: https://doi.org/10.5009/gnl15175

Kozlovska T. I., Sander S. V., Zlepko S. M., Vasilenko V. B., Pavlov V. S., Klapouschak A. Yu., Dumenko V. P., Maciejewski M., Dzierżak R., Surtel W. Device to determine the level of peripheral blood circulation and saturation. Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments. - International Society for Optics and Photonics. 2016. 100312Z-100312Z-6

Abugo O. O., Herman P., Lakowicz J. R. Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood. J. Biomed. Opt. 2001. Vol. 6, № 3. Р. 359-365. DOI: https://doi.org/10.1117/1.1381053

Suhling K., Siegel J., Phillips D., French P. M., Leveque-Fort S., D Webb S. E., Davis D. M. Imaging the environment of green fluorescent protein. Biophys. J. 2002. Vol. 83. Р. 3589-3595. DOI: https://doi.org/10.1016/S0006-3495(02)75359-9

Gannot I., Garashi A., Gannot G., Chernomordik V., Gandjbakhche A. In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers. Appl. Opt. 2003. Vol. 42 (16). Р. 3073-3080. DOI: https://doi.org/10.1364/AO.42.003073

Думенко В. П. Сучасні лазерні люмінесцентні методи дослідження злоякісних новоутворень в біологічних тканинах. Сучасні проблеми фізико-математичної освіти і науки: збірник матеріалів конференції (Київ, 25-26 травня 2017 року). Київ: НПУ ім. М. П. Драгоманова. С. 27-28.

Soukos N. S., Som S., Abernethy A. D., Ruggiero K., Lee J. , Dunham C., Doukas A. G., Goodson J. M., Phototargeting oral black-pigmented bacteria. Antimicrob. Agents Chemother. 2005. Vol. 49. Р. 1391-1396. DOI: https://doi.org/10.1128/AAC.49.4.1391-1396.2005

Schneckenburger H., König K., Dienersberger T., Hahn R. Time-gated microscopic imaging and spectroscopy in medical diagnosis and photobiology. Opt. Eng. 1994. Vol. 33. Р. 3156-3167. DOI: https://doi.org/10.1117/12.177101

Published

2024-10-17

Issue

Section

Actual problems of physics and astronomy

How to Cite

Methods of low-energy induced fluorescence and spectrophotometry for the study of blood cells. (2024). Mathematics, Informatics, Physics: Science and Education, 1(2), 129-137. https://doi.org/10.31652/3041-1955/2024-01-02-04